Stochastic kinetic study of protein aggregation and molecular crowding effects of Aβ40 and Aβ42
Bridstrup, J., Yuan, J., Schreck, J. S.. (2022). Stochastic kinetic study of protein aggregation and molecular crowding effects of Aβ40 and Aβ42. Journal of the Chinese Chemical Society, doi:10.1002/jccs.202200365
| Title | Stochastic kinetic study of protein aggregation and molecular crowding effects of Aβ40 and Aβ42 |
|---|---|
| Author(s) | John Bridstrup, Jian‐Min Yuan, John S. Schreck |
| Abstract | Two isoforms of beta-amyloid peptides, A beta 40 and A beta 42, differ from each other only in the last two amino acids, IA, at the end of A beta 42. They, however, differ significantly in their ability in inducing Alzheimer's disease (AD). The rate curves of fibril growth of A beta 40 and A beta 42 and the effects of molecular crowding have been measured in in vitro experiments. These experimental curves, on the other hand, have been fitted in terms of rate constants for elementary reaction steps using rate equation approaches. Several sets of such rate parameters have been reported in the literature. Employing a recently developed stochastic kinetic method, implemented in a browser-based simulator, popsim, we study to reveal the differences in the kinetic behaviors implied by these sets of rate parameters. In particular, the stochastic method is used to distinguish the kinetic behaviors between A beta 40 and A beta 42 isoforms. As a result, we make general comments on the usefulness of these sets of rate parameters. |
| Publication Title | Journal of the Chinese Chemical Society |
| Publication Date | Oct 10, 2022 |
| Publisher's Version of Record | https://dx.doi.org/10.1002/jccs.202200365 |
| OpenSky Citable URL | https://n2t.net/ark:/85065/d7pv6q55 |
| OpenSky Listing | View on OpenSky |
| CISL Affiliations | TDD, AIML |